Neuropsychiatry in World War II

detailed suggestions in the use of various methods that are not possible to transmit through research publications. The subjects covered in Volume XI include nearly all the techniques that would be needed in a thorough study of enzyme structure. Subjects that would appear to be missing generally have been covered by articles in previous volumes. The first section contains ten articles on amino acid analysis and related procedures. The primary technique treated is that of automatic amino acid analysis, although certain specific amino acid determinations are included. Four articles on end-group analysis and four articles on methods of subunit separation follow. The fourth and fifth sections deal with techniques for cleavage of disulfide and peptide bonds, respectively. They are followed by 11 articles on separation of peptides and eight articles on sequence determination. The eighth section includes 25 articles on general techniques for modification of enzyme structure. These cover a very broad range, from oxidation with hydrogen peroxide to limited proteolysis by exopeptidase. Seven articles describe techniques specific for certain enzymes or enzyme classes. The last section brings together a number of techniques classified as being suitable for investigation of conformation changes, although in general they are of much broader significance. These techniques include titration, hydrogen exchange, difference spectroscopy, fluorescence, reporter groups, dialysis and diffusion, and immunological approaches. The broader nature of the topics covered make these articles more comprehensive than those in the preceding sections. In particular, the very comprehensive article on fluorescence measurements by L. Brand and B. Witholt is worthy of special note. From this summary of the contents of this volume it should be apparent that the order of presentation is most pertinent, following the logical order in which the techniques would be used upon purification of a new enzyme. Moreover, the volume may be of greater general value than previous volumes because of the general nature of the techniques presented. Indeed, of the 94 articles in the volume, nine are limited to use for only a small class of enzymes. The remaining 85 articles would be of use to investigators concerned with any aspect of enzyme structure.